human oral squamous cell carcinoma oscc cell lines scc15  (ATCC)

Journal: BMC Complementary and Alternative Medicine

Article Title: Aloe-emodin induces apoptosis in human oral squamous cell carcinoma SCC15 cells

doi: 10.1186/s12906-018-2353-z

Figure Lengend Snippet: Effects of indicated compounds on the cell viability of the SCC15 cells. a Exponentially growing of SCC15 cells was treated with aloe-emodin, rhein and physcion in various concentrations (0, 12.5, 25, 50, 100 and 200 μM) for 48 h. b SCC15 cells were treated with 12.5, 25, 50, 100 and 200 μM of aloe-emodin for various times (24 h, 48 h, 72 h). The cell viability was determined using MTT assay. Each bar represents the mean ± SD

Article Snippet: Human oral squamous cell carcinoma cell line SCC15 was donated by Zhang Xin-yan, professor of Capital medical university school of stomatology (Beijing, China), which was obtained from the American Type Culture Collection (Manassas VA) and stored in our laboratory.

Techniques: MTT Assay

Journal: BMC Complementary and Alternative Medicine

Article Title: Aloe-emodin induces apoptosis in human oral squamous cell carcinoma SCC15 cells

doi: 10.1186/s12906-018-2353-z

Figure Lengend Snippet: Aloe-emodin induces apoptosis of the SCC15 cells. SCC15 cells were treated by aloe-emodin of 0 μM ( a and c ) and 50 uM ( b and d ) for 24 h and 48 h, respectively. Annexin binding and propidium iodide (PI) staining were analyzed by FACScan. Q4: viable cells; Q3: early apoptotic cells; Q2: late apoptotic cells; Q1: death cells. The graph visualizes early and late stage apoptotic cells ( e ). Results were expressed as the mean ± SD ( n = 3) and analyzed by Students’ t -test ( *P < 0.05 , **P < 0.01)

Article Snippet: Human oral squamous cell carcinoma cell line SCC15 was donated by Zhang Xin-yan, professor of Capital medical university school of stomatology (Beijing, China), which was obtained from the American Type Culture Collection (Manassas VA) and stored in our laboratory.

Techniques: Binding Assay, Staining

Journal: BMC Complementary and Alternative Medicine

Article Title: Aloe-emodin induces apoptosis in human oral squamous cell carcinoma SCC15 cells

doi: 10.1186/s12906-018-2353-z

Figure Lengend Snippet: Western blotting analyses of aloe-emodin on the expression of apoptotic proteins. a SCC15 cells were treatment with various concentrations (0, 25 and 50 μM) of aloe-emodin for 48 h and the levels of caspase-9, caspase-3 were measured by Western blotting analysis. Increased expression of caspase-9 and caspase-3 were observed. β-actin was used as a loading control. b Densitometry analysis of Western blots, Data were expressed as the mean ± SD ( n = 3) and analyzed by one-way ANOVA using Dunnett’s multiple-comparison test ( *P < 0.05 , **P < 0.01)

Article Snippet: Human oral squamous cell carcinoma cell line SCC15 was donated by Zhang Xin-yan, professor of Capital medical university school of stomatology (Beijing, China), which was obtained from the American Type Culture Collection (Manassas VA) and stored in our laboratory.

Techniques: Western Blot, Expressing, Control, Comparison